The trailer opens with Eddie Brock (Topher Grace) entering a church, kneeling at a pew and praying for God to kill Peter Parker. Cut to fun and energetic footage of a black suit wearing Spider-Man swinging through the city with a voiceover from Parker (Tobey Maguire) noting how the suit seems to give him more power. Note: The acrobatics end as Spider-Man plows into the Sandman (Thomas Haden Church).
The next scene is Parker with Dr. Curt Conners (Dylan Baker), who is examining a piece of the black suit. The scientist notes that the substance is very "responsive to aggression." At that exact moment, the piece of suit slithers toward Parker but Conners traps it in a beaker. "It seems to like you," adds Conners.
The audience then catches a glimpse of Harry Osborn (James Franco) looking into a mirror and seeing the face of his father, Norman (Willem Dafoe), looking back at him. The elder Osborn's voice implores his son, "You know what you have to do." Cut to Harry, in modified Green Goblin gear (the likes of which make him look like a high tech X-gamer), chasing Parker through the city. Parker is in plain clothes, but is swinging on webs and using his Spidey powers… trying to get away from his former friend. Harry throws bladed-boomerangs, similar to what the original Green Goblin used in the first Spider-Man ("I hate these things," quips Parker), and swings some sort of lightsaber-looking green bat. A shot of Mary Jane (Kirsten Dunst) asking Parker what's happening to him — alluding to the fact that he is becoming a different person with the suit — is dropped in right before the next sequence, which shows an angry Parker (with the black suit clearly visible under his dress shirt) walking among from Harry who is laying on the ground. Harry throws a pumpkin bomb at him, but Parker quickly flips around, catches the bomb and throws it back. The explosion does some serious damage to Harry.(only thing I don't remember but it probably was there.)
The next scene shows Parker reaching out to M.J. asking for her help. Cut to black suit Spider-Man in a bell tower trying to rip the costume off. This is where the trailer comes full circle, as the bell tower is in the same church where Brock is praying. He looks up at the commotion and realizes that Parker is Spider-Man. Pieces of the suit begin to fall from the tower. Some lands on Brock's jacket; he can't shake the substance off so he takes off his jacket. More falls directly onto his skin; again, he can't shake it off. Finally, the bulk of the suit falls onto Brock and the vicinity around him. It consumes the man like a slimy blob. Suddenly, Brock looks up and it's the Venom that we all know — slithery, pointed white eyes and sharp fangs. The creature jumps at the camera then… cut to black.
One final sequence is shown. Spider-Man, clearly in the throws of a massive battle (his traditional red and blue costume is ripped to shreds, and half of Parker's face is exposed), is looking around frantically trying to find the villain he's fighting. But he can't see or Spidey sense anyone. Suddenly, Venom's claws reach down from above him, grab him by the head and pull him up and out of the shot. End trailer.